Journal: Frontiers in Immunology

Article Title: Revealing the significance of tissue-resident memory T cells in lung adenocarcinoma through bioinformatic analysis and experimental validation

doi: 10.3389/fimmu.2025.1600863

Figure Lengend Snippet: Validation of the protein expression levels of genes involved in the lung T RM -related prognostic signature. (A) RNA expression levels of the genes included in the prognostic model in LUAD and adjacent normal tissues. The abscissa axis shows the gene names, and the vertical axis shows the RNA expression levels. (B–J) Immunohistochemical staining images obtained from the Human Protein Atlas database ( https://www.proteinatlas.org/ ): (B) SLC16A3 , (C) ARHGAP11A , (D) PTTG1 , (E) GPRIN1 , (F) TYMS , (G) HLA-DQA1 , (H) ALOX5AP and (J) OASL . The names of the genes and antibodies are presented at the top of each panel. The left image of each panel is the adjacent normal tissue, whereas the right image is the LUAD tissue. The staining intensity is labeled under each image.

Article Snippet: Primary polyclonal antibodies against TYMS (15047-1-AP, ProteinTech) and β-Actin (66009-1-Ig, ProteinTech) were used at dilutions of 1:3,000 and 1:20,000, respectively.

Techniques: Biomarker Discovery, Expressing, RNA Expression, Immunohistochemical staining, Staining, Labeling

Journal: Frontiers in Immunology

Article Title: Revealing the significance of tissue-resident memory T cells in lung adenocarcinoma through bioinformatic analysis and experimental validation

doi: 10.3389/fimmu.2025.1600863

Figure Lengend Snippet: Validation of the prognostic significance of the genes involved in the lung T RM -related prognostic signature via proteomic data. K–M curves showing the survival status of LUAD patients with low and high protein expression of (A) SLC16A3 , (B) TYMS , (C) HLA-DQA1 , (D) ALOX5AP and (E) OASL .

Article Snippet: Primary polyclonal antibodies against TYMS (15047-1-AP, ProteinTech) and β-Actin (66009-1-Ig, ProteinTech) were used at dilutions of 1:3,000 and 1:20,000, respectively.

Techniques: Biomarker Discovery, Expressing

Journal: Frontiers in Immunology

Article Title: Revealing the significance of tissue-resident memory T cells in lung adenocarcinoma through bioinformatic analysis and experimental validation

doi: 10.3389/fimmu.2025.1600863

Figure Lengend Snippet: Validation of the clinical and biological roles of TYMS through experiments on LUAD clinical samples (n=30) and cell lines. (A) Images of LUAD samples with negative TYMS expression (A1) , low TYMS expression (A2) , moderate TYMS expression (A3) and high TYMS expression (A4) in the immunohistochemical experiment. (B) Kaplan–Meier curves showing the survival status of LUAD patients with negative and positive TYMS staining. (C) Percentage plot showing the proportion of samples with negative and positive staining among tumors at the M0 and M1 stages. (D) Percentage plot showing the proportions of samples with negative and positive staining among patients with clinical stages II+III and clinical stage IV disease. (E) Column chart showing the relative mRNA expression levels of TYMS in the si- TYMS -1#, si- TYMS -2# and control groups via qRT–PCR. (F) Western blotting results showing the protein levels of TYMS in the si- TYMS -1#, si- TYMS -2# and control groups. (G) The corresponding grayscale of the WB results in (F) . (H) Proliferation curves of H1395 cells in the si- TYMS -1#, si- TYMS -2# and control groups. (I) Images of H1395 cells in the si- TYMS -1#, si- TYMS -2# and control groups captured by the IncuCyte platform 120 hours after seeding into 96-well plates.

Article Snippet: Primary polyclonal antibodies against TYMS (15047-1-AP, ProteinTech) and β-Actin (66009-1-Ig, ProteinTech) were used at dilutions of 1:3,000 and 1:20,000, respectively.

Techniques: Biomarker Discovery, Expressing, Immunohistochemical staining, Staining, Control, Quantitative RT-PCR, Western Blot

Journal: OncoTargets and Therapy

Article Title: Exploring Protein Expression Profiles in Lung Cancer Insufficient Microwave Ablation: Implications for Recurrence

doi: 10.2147/OTT.S508577

Figure Lengend Snippet: Differential Expression Proteins

Article Snippet: The primary antibodies used in our study were listed below CTP synthase 1 (CTPS1) (Boster #A06374-2, 1:200), Thymidylate synthetase (TYMS) (Boster #BM5360, 1:100), NME nucleoside diphosphate kinase 3 (NME3) (Proteintech #15136-1-AP, 1:200), Renin binding protein (RENBP) (Bioss #bs-8497R, 1:200), Fucose kinase (FCSK) (Sangon #D127113, 1:300), Pyridoxal phosphatase (PDXP) (Abclonal #A17455, 1:300), and Sulfite oxidase (SUOX) (Proteintech #15075-1-AP, 1:200).

Techniques: Quantitative Proteomics, Binding Assay

Journal: OncoTargets and Therapy

Article Title: Exploring Protein Expression Profiles in Lung Cancer Insufficient Microwave Ablation: Implications for Recurrence

doi: 10.2147/OTT.S508577

Figure Lengend Snippet: Relationship Between DEPs and Prognosis From TCGA Dataset

Article Snippet: The primary antibodies used in our study were listed below CTP synthase 1 (CTPS1) (Boster #A06374-2, 1:200), Thymidylate synthetase (TYMS) (Boster #BM5360, 1:100), NME nucleoside diphosphate kinase 3 (NME3) (Proteintech #15136-1-AP, 1:200), Renin binding protein (RENBP) (Bioss #bs-8497R, 1:200), Fucose kinase (FCSK) (Sangon #D127113, 1:300), Pyridoxal phosphatase (PDXP) (Abclonal #A17455, 1:300), and Sulfite oxidase (SUOX) (Proteintech #15075-1-AP, 1:200).

Techniques:

Journal: OncoTargets and Therapy

Article Title: Exploring Protein Expression Profiles in Lung Cancer Insufficient Microwave Ablation: Implications for Recurrence

doi: 10.2147/OTT.S508577

Figure Lengend Snippet: Differential expression proteins of nucleotide metabolism for predicting survival outcomes. The Kaplan-Meier survival analysis and COX regression analysis were conducted on the CTPS1 ( A ) and TYMS ( B ) using data from the TCGA dataset. ( C ) The distribution of risk scores, survival analysis, and heatmap visualization for patients with a double-gene signature. Upper left: The scatter plot represented the risk score from low to high. Different colors represented different groups. Upper right: Kaplan-Meier survival analysis of the risk model from dataset, comparison among different groups was made by Log rank test. Lower left: the scatter plot distribution represented the risk score of different samples corresponding to the survival time and survival status. The bottom heatmap was the gene expression from the signature. Lower right: the ROC curve of the genes.

Article Snippet: The primary antibodies used in our study were listed below CTP synthase 1 (CTPS1) (Boster #A06374-2, 1:200), Thymidylate synthetase (TYMS) (Boster #BM5360, 1:100), NME nucleoside diphosphate kinase 3 (NME3) (Proteintech #15136-1-AP, 1:200), Renin binding protein (RENBP) (Bioss #bs-8497R, 1:200), Fucose kinase (FCSK) (Sangon #D127113, 1:300), Pyridoxal phosphatase (PDXP) (Abclonal #A17455, 1:300), and Sulfite oxidase (SUOX) (Proteintech #15075-1-AP, 1:200).

Techniques: Quantitative Proteomics, Comparison, Gene Expression

Journal: Frontiers in Immunology

Article Title: Single-cell sequencing analysis reveals cancer-associated pericyte subgroup in esophageal squamous cell carcinoma to predict prognosis

doi: 10.3389/fimmu.2024.1474673

Figure Lengend Snippet: Multiple fluorescence staining of tissues samples from ESCC patients. (A, B) Validation of high and low expression level of c5_TYMS in ESCC tumor tissues samples and spatial distribution with microphages and fibroblasts. (C, D) Validation of high and low expression of level c4_SOD2 in ESCC tumor tissue samples and spatial distribution with microphages and fibroblasts.

Article Snippet: Incubate overnight at 4°C with purified rabbit anti-human PDGFRβ (ab32570, 1:100, Abcam, USA), rabbit anti-human SOD2 (66474-1-Ig, 1:300, Proteintech, Wuhan, China), rabbit anti-human CD68 (66231-2-Ig, 1:2000, Proteintech, Wuhan, China), rabbit anti-human TYMS (66725-1-Ig, 1:200, Proteintech, Wuhan, China), mouse anti-human α-SMA(67735-1-Ig, 1:400, Proteintech, Wuhan, China) and purified rabbit anti-human EPCAM (21050-1-AP, 1:1000, Proteintech, Wuhan, China).

Techniques: Fluorescence, Staining, Biomarker Discovery, Expressing

Journal: Frontiers in Immunology

Article Title: Single-cell sequencing analysis reveals cancer-associated pericyte subgroup in esophageal squamous cell carcinoma to predict prognosis

doi: 10.3389/fimmu.2024.1474673

Figure Lengend Snippet: Docking results of available proteins with small molecules.

Article Snippet: Incubate overnight at 4°C with purified rabbit anti-human PDGFRβ (ab32570, 1:100, Abcam, USA), rabbit anti-human SOD2 (66474-1-Ig, 1:300, Proteintech, Wuhan, China), rabbit anti-human CD68 (66231-2-Ig, 1:2000, Proteintech, Wuhan, China), rabbit anti-human TYMS (66725-1-Ig, 1:200, Proteintech, Wuhan, China), mouse anti-human α-SMA(67735-1-Ig, 1:400, Proteintech, Wuhan, China) and purified rabbit anti-human EPCAM (21050-1-AP, 1:1000, Proteintech, Wuhan, China).

Techniques: Binding Assay

Journal: Frontiers in Immunology

Article Title: Single-cell sequencing analysis reveals cancer-associated pericyte subgroup in esophageal squamous cell carcinoma to predict prognosis

doi: 10.3389/fimmu.2024.1474673

Figure Lengend Snippet: Molecular docking results of predicted drugs and protein encoded by target genes. (A) PDGFRβ and docetaxel. (B) TYMS and docetaxel. (C) PDGFRβ and cytarabine. (D) TYMS and cytarabine. (E) PDGFRβ and bisindolylmalemide. (F) SOD2 and bisindolylma-leimide. (G) PDGFRβ and raloxifene. (H) SOD2 and raloxifene.

Article Snippet: Incubate overnight at 4°C with purified rabbit anti-human PDGFRβ (ab32570, 1:100, Abcam, USA), rabbit anti-human SOD2 (66474-1-Ig, 1:300, Proteintech, Wuhan, China), rabbit anti-human CD68 (66231-2-Ig, 1:2000, Proteintech, Wuhan, China), rabbit anti-human TYMS (66725-1-Ig, 1:200, Proteintech, Wuhan, China), mouse anti-human α-SMA(67735-1-Ig, 1:400, Proteintech, Wuhan, China) and purified rabbit anti-human EPCAM (21050-1-AP, 1:1000, Proteintech, Wuhan, China).

Techniques: